Everything about Acridine Orange totally explained
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Acridine orange is a
nucleic acid selective
fluorescent cationic
dye useful for cell cycle determination. It is cell-permeable, and interacts with
DNA and
RNA by
intercalation or
electrostatic attractions. When bound to DNA, it's very similar spectrally to
fluorescein, with an
excitation maximum at 502 nm and an
emission maximum at 525 nm (green). When it associates with RNA, the excitation maximum shifts to 460 nm (blue) and the emission maximum shifts to 650 nm (red). The dye is often used in
epifluorescence microscopy.
Acridine orange is prepared from
coal tar and
creosote oil.
Acridine orange can be used in conjunction with
ethidium bromide to differentiate between live and apoptotic cells.
Further Information
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